In accordance with ARRIVE guidelines, the French Institution animal ethics committee approval was obtained for the protocols used on animals. Orthotopic human glioblastoma xenografts were established in 4-6 weeks-old female nude mice (Janvier) with 2.5 × 105 cells as previously described [23 (link)]. Each GSC line was xenografted at least in three mice. Mice were sacrificed at the appearance of neurological signs. Immunohistochemistry analysis was performed on the excised brains on paraffin-embedded sections (5 μm) [23 (link)].
Boyrie S., Delmas C., Lemarié A., Lubrano V., Dahan P., Malric L., Luis J., Gilhodes J., Tosolini M., Mouly L., Lehmann M., Toulas C., Cohen-Jonathan Moyal E, & Monferran S. (2018). RND1 regulates migration of human glioblastoma stem-like cells according to their anatomical localization and defines a prognostic signature in glioblastoma. Oncotarget, 9(73), 33788-33803.
Other organizations :
Institut universitaire du cancer de Toulouse Oncopole, Centre Hospitalier Universitaire de Toulouse, Université Toulouse III - Paul Sabatier, Institut National de Recherche en Santé Publique, Institut de Neurophysiopathologie, Institut Claudius Regaud, Centre de Recherche en Cancérologie de Toulouse, Laboratoire de Biophotonique et Pharmacologie
Cell line (at least three GSC lines were xenografted)
dependent variables
Tumor growth (measured by appearance of neurological signs)
control variables
Age of female nude mice (4-6 weeks old)
Number of cells used for xenografts (2.5 × 10^5 cells)
Immunohistochemistry analysis on excised brains
controls
Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned
Annotations
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