Psoriasin Overexpression and Silencing

The full-length coding sequence of psoriasin [GenBank: AJ012825.1] was cloned into pcDNA3.1 [37 (link)] and transfected into TU167 cells by Lipofectamine (Invitrogen, Burlington, ON, Canada). Stable clones were selected by zeocin, as a selection marker. Several clones were developed, and immunoblot determined protein expression of psoriasin. TU167-mock was generated by transfection with the empty vector. JMAR cells were also transfected with protein-specific siRNA (Qiagen, Mississauga, ON, Canada) of psoriasin. siRNA was conducted using Dharmafect-4 reagent (Dharmacon).

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Dey K.K., Sarkar S., Pal I., Das S., Dey G., Bharti R., Banik P., Roy J., Maity S., kulavi I, & Mandal M. (2015). Mechanistic attributes of S100A7 (psoriasin) in resistance of anoikis resulting tumor progression in squamous cell carcinoma of the oral cavity. Cancer Cell International, 15, 74.

Publication 2015

Lipofectamine Dharmafect-4 reagent

Cells Clones Coding sequence Immunoblot Lipofectamine Protein Psoriasin Sirna Transfection Vector Zeocin

Corresponding Organization :

Other organizations : Indian Institute of Technology Kharagpur, Dr. R. Ahmed Dental College and Hospital, Medical College and Hospital, Kolkata, Bankura Sammilani Medical College

Top 5 similar protocols

Variable analysis

independent variables

Transfection of psoriasin coding sequence into TU167 cells
Transfection of protein-specific siRNA of psoriasin into JMAR cells

dependent variables

Protein expression of psoriasin

control variables

TU167-mock cells (transfected with empty vector)

controls

Positive control: Stable clones expressing psoriasin
Negative control: TU167-mock cells (transfected with empty vector)

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