Bioluminescence Imaging using Luciferase

Bioluminescence by luciferase was observed using Luminoview LV200 (Olympus, Osaka, Japan). Luminescence was acquired in MetaMorph^® imaging system (Molecular Devices, Sunnyvale, CA). Pv11 cells were attached onto a 35 mm glass bottom dish (AGC Techno Glass Co., Ltd., Tokyo, Japan) using 50 µg/ml cationic polymer, Polyethyleneimine “Max” MW 40000 (Polysciences, Inc., Warrington, PA). The cationic polymer was prepared as described previously^{41 (link)}. D-luciferin potassium salt (Wako Pure Chemical Industries, Osaka, Japan) was dissolved in distilled water and kept at 4 °C in the dark until use.

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Kikuta S., Watanabe S.J., Sato R., Gusev O., Nesmelov A., Sogame Y., Cornette R, & Kikawada T. (2017). Towards water-free biobanks: long-term dry-preservation at room temperature of desiccation-sensitive enzyme luciferase in air-dried insect cells. Scientific Reports, 7, 6540.

Publication 2017

Luminoview LV200 MetaMorph 35 mm glass bottom dish Polyethyleneimine “Max” MW 40000 D-luciferin potassium salt

Cationic Cells Devices Dish Luciferase Luciferin Luminescence Polyethyleneimine Polymer Potassium Salt

Corresponding Organization : Tokyo University of Agriculture and Technology

Other organizations : Kazan Federal University, Institute of Agrobiological Sciences

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Variable analysis

independent variables

Cationic polymer (Polyethyleneimine "Max" MW 40000) concentration (50 µg/ml)

dependent variables

Bioluminescence by luciferase

control variables

Pv11 cells attached onto a 35 mm glass bottom dish
D-luciferin potassium salt dissolved in distilled water and kept at 4 °C in the dark

controls

Positive control: Not specified
Negative control: Not specified

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