Quantifying Cannabinoid Levels in Serum

To validate that there was a dose-dependent change in CBD levels, serum levels of cannabinoids were analyzed [22 (link)]. In brief, methanolic extracts of 90 µl of serum were partially purified on C18 solid phase extraction columns (Zorbax) and eluants were analyzed using HPLC/MS/MS (API 3000, Applied Biosystems). Deuterium-labeled anandamide elutes in the same fraction as CBD and was used as an internal standard to monitor recovery. Levels of CBD and THC were analyzed using standard curves with Analyst Software as previously described [22 (link)]. During analysis it was discovered that each of the samples contained a small fraction THC in addition to CBD. This can occur during synthesis and is often unknown if the levels are not analyzed. The plasma ratio in each dose was ca 25:1 CBD:THC (Additional file 1: Figure S3B).

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Sadaka A.H., Ozuna A.G., Ortiz R.J., Kulkarni P., Johnson C.T., Bradshaw H.B., Cushing B.S., Li A.L., Hohmann A.G, & Ferris C.F. (2021). Cannabidiol has a unique effect on global brain activity: a pharmacological, functional MRI study in awake mice. Journal of Translational Medicine, 19, 220.

Publication 2021

API 3000

Anandamide Cannabinoids Deuterium Hplc Methanolic Ms ms Plasma Serum Solid phase extraction Synthesis

Corresponding Organization : Northeastern University

Other organizations : The University of Texas at El Paso, Indiana University Bloomington

Top 5 similar protocols

Variable analysis

independent variables

Dose of CBD

dependent variables

Serum levels of CBD
Serum levels of THC

control variables

Methanolic extraction of 90 µl of serum
Partial purification on C18 solid phase extraction columns (Zorbax)
HPLC/MS/MS (API 3000, Applied Biosystems) analysis
Deuterium-labeled anandamide as internal standard to monitor recovery
Standard curves with Analyst Software

controls

Deuterium-labeled anandamide as a positive control to monitor recovery

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