Asthma Mouse Model Generation

The generation of an asthma mouse model was established as previously described (29 (link)). Briefly, both male and female mice were sensitized and challenged every other week for 6 weeks with intranasal administrations of 25 μg Der f 1 (Indoor Biotechnologies) under isoflurane anesthesia. BaP (Sigma-Aldrich) was dissolved in borate saline buffer (Sigma-Aldrich) and intranasally administered at a concentration of 20 μM once every week during Der f 1 sensitization and challenge. In some cases, the mice were pre-treated with Fasudil or Y-27632 dissolved in 0.5% DMSO in phosphate-buffered saline (PBS) at a dose of 30 mg/ml by intraperitoneal administration 1 h before every single BaP treatment. Age- and gender-matched control mice were treated with PBS.