Comprehensive Neurodegeneration Biomarker Analysis

Anti-p-tau S202 (ab108387, 1:1000), p-tau S396 (ab109390, 1:1000), p-GSK3β Y216 (ab75745, 1:1000), GSK3β (ab32391, 1:1000), and p-RhoA S188 (ab41435, 1:500) were purchased from Abcam (Cambridge, UK). Anti-GST (10,000-0-AP, 1:5000), GFP (66002-2-Ig, 1:5000), His (66005-1-Ig, 1:5000), RhoA (10749-1-AP, 1:500), GAPDH (60004-1-Ig, 1:10,000), and TG-2 (60044-1-Ig, 1:500), TG-2 (10234-2-AP, 1:500), anti-human IgG (16402-1-AP, 1:200 for immunofluorescence, 1:1000 for western blot) were purchased from Proteintech. The anti-TG-2 (sc-166697, 1:200) antibody was from Santa Cruz. Tau5 antibody (AHB0042, 1:1000), anti-p-Tau (Ser202, Thr205) (MN1020, 1:1000), anti-p-tau T181 (MN1050, 1:1000), Goat anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (A-11001, 1:1000), Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (A-11034, 1:1000), Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (A-11012, 1:1000), Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (A-11005, 1:1000) were purchased from ThermoFisher. Anti p-tau S404 (310196, 1:1000), and p-tau T231 (381181, 1:1000) were from Zenbio. Cell-permeable Rho inhibitor (C3 Trans based) (CT04-A), RhoA activator (Cytoskeleton, CN01), and Rhotekin RBD protein on GST beads (RT02-A) were purchased from Cytoskeleton. Y-27632 (sc-3536) was purchased from Santa Cruz Biotechnology. Protein A MagBeads (L00464) were purchased from GenScript. TREM2 ELISA kits (SEK11084) were purchased from Sino Biological.