Quantification of Gene Expression

RNA was isolated using Gene MATRIX Universal RNA/miRNA Purification Kit (EURx) and transcribed to cDNA with Transcriptor Universal cDNA Master (Roche). Primer sequences are given in Table S2. Transcript abundance was measured using iTaq Universal SYBR Green Supermix (Bio-Rad) and LightCycler 480. 18S RNA was used as a housekeeping control gene. Relative transcript abundance was assessed using the 2^-ΔΔCT method as previously described [30 (link)].

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Białopiotrowicz-Data E., Noyszewska-Kania M., Jabłońska E., Sewastianik T., Komar D., Dębek S., Garbicz F., Wojtas M., Szydłowski M., Polak A., Górniak P, & Juszczyński P. (2023). SIRT1 and HSP90α feed-forward circuit safeguards chromosome segregation integrity in diffuse large B cell lymphomas. Cell Death & Disease, 14(10), 667.

Publication 2023

Gene MATRIX Universal RNA/miRNA Purification Kit Transcriptor Universal cDNA Master iTaq Universal SYBR Green Supermix LightCycler 480

18s rna Cdna Gene Housekeeping gene Mirna Primer Sybr green

Corresponding Organization :

Other organizations : Instytut Hematologii i Transfuzjologi

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Transcript abundance

control variables

18S RNA used as a housekeeping control gene

controls

Positive control: None mentioned
Negative control: None mentioned

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