Transfection of Human Neuroblastoma Cells

Human neuroblastoma SH-SY5Y cells (ATCC, Manassas, VA, USA) were grown in complete media containing DMEMs, 10% fetal bovine serum (vol/vol), and antibiotics (penicillin-streptomycin 100 U/mL, Thermo Fisher Scientific, Waltham, MA, USA). The atmospheric condition used to preserve the cells consisted of 5% CO₂/95% air at 37 °C and maintained a humidified environment. X-tremeGENE HP transfection reagents (Roche, Basel, Switzerland) were used for transient transfection of DNA constructs (pCMV-tTA, TetP-β23, and pTet-dual2 [5 (link)]) following the manufacturer’s instructions.

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Kim H., Maeng H.J., Kim J.H., Yoon J.H., Oh Y., Paek S.M, & Lee Y. (2022). Synthetic Peucedanocoumarin IV Prevents α-Synuclein Neurotoxicity in an Animal Model of Parkinson’s Disease. International Journal of Molecular Sciences, 23(15), 8618.

Publication 2022

SH-SY5Y cells fetal bovine serum penicillin-streptomycin X-tremeGENE HP transfection reagents

Antibiotics Cells Dna constructs Fetal bovine serum Human Neuroblastoma Penicillin Streptomycin Transfection Transient

Corresponding Organization : Gyeongsang National University

Other organizations : Gachon University, Hanyang University

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Variable analysis

independent variables

DNA constructs (pCMV-tTA, TetP-β23, and pTet-dual2)

dependent variables

Not explicitly mentioned

control variables

Cell type (human neuroblastoma SH-SY5Y cells)
Culture medium (DMEM with 10% fetal bovine serum and antibiotics)
Atmospheric conditions (5% CO2/95% air at 37°C with humidified environment)

controls

Positive control: Not specified
Negative control: Not specified

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