Histological Scoring of GVHD in Mice

Clinical and histologic scoring of mice was monitored as previously reported [19 (link), 27 (link)]. The survival, body weight, and appearance of mice in each group were assessed before sacrifice at the indicated time point. The clinical score of GVHD was evaluated using a clinical scoring system as shown in Additional file 4: Figure S4a. For histologic scoring of mice, the sections of the liver, lung, and skin were stained with hematoxylin-eosin (H&E) staining and observed under a Nikon ElipseTi-U microscope (Nikon, Tokyo, Japan) as we described before [26 (link)]. Each pathological index of the lung, liver, or skin was graded from 0 to 3, and the total GVHD histologic index was the sum of the parameters listed in Additional file 5: Table S1.

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Zhao Q., Zhang L., Wei Y., Yu H., Zou L., Huo J., Yang H., Song B., Wei T., Wu D., Zhang W., Zhang L., Liu D., Li Z., Chi Y., Han Z, & Han Z. (2019). Systematic comparison of hUC-MSCs at various passages reveals the variations of signatures and therapeutic effect on acute graft-versus-host disease. Stem Cell Research & Therapy, 10, 354.

Publication 2019

ElipseTi-U microscope

Body weight Eosin Liver Lung Mice Microscope Skin

Corresponding Organization :

Other organizations : Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming Medical University, First Affiliated Hospital of Kunming Medical University, Affiliated Hospital of Southwest Medical University, Soochow University, First Affiliated Hospital of Soochow University, Jinan University, Nankai University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Survival
Body weight
Appearance of mice
Clinical score of GVHD
Histologic score of GVHD in liver, lung, and skin

control variables

None explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

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