The biodistribution of intravenously (i.v.)-injected 111In-labelled L2/3, L2/3:ox-MWNTs, and L2/3:ox-MWNTs-NH3+ was firstly examined by 3D whole body SPECT/CT imaging. Mice were injected via tail vein with 100 μg of labelled 111In(L2/3):f-MWNTs (1 mg/ml in PBS) or In(L2/3) alone (100 μg) under isoflurane anaesthesia. SPECT/CT scans were carried out on the same animal at multiple time points: immediately after injection, at 4 h and 24 h. Mice were placed in the prone position and SPECT scans were acquired over 24 projections (60 s per projection), using a 4-head scanner with 1.4 mm pinhole collimators, for a total acquisition time of 30-40 min (Nano-SPECT/CT, USA) 27 (link). CT scans (45 kVP X-ray) were performed after each SPECT. All images were reconstructed by MEDISO software (Medical Imaging Systems), and SPECT and CT images were merged using the VivoQuantTM software (inviCRO, USA).
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Biodistribution of Radiolabeled Nanomaterials
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Other organizations : King's College London, Centre de Biophysique Moléculaire, Queen Mary University of London
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Variable analysis
independent variables
- 111In-labelled L2/3
- L2/3:ox-MWNTs
- L2/3:ox-MWNTs-NH3+
- Biodistribution of 111In-labelled compounds
- Injection of 100 μg of labelled 111In(L2/3):f-MWNTs (1 mg/ml in PBS) or In(L2/3) alone (100 μg) under isoflurane anaesthesia
- SPECT/CT scans carried out on the same animal at multiple time points: immediately after injection, at 4 h and 24 h
- Mice placed in the prone position for SPECT scans acquired over 24 projections (60 s per projection), using a 4-head scanner with 1.4 mm pinhole collimators, for a total acquisition time of 30-40 min
- CT scans (45 kVP X-ray) performed after each SPECT
- Positive control: 111In(L2/3):f-MWNTs
- Negative control: In(L2/3) alone
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