Immunoblotting of AEG-1, EZH2, and GAPDH

For immunoblotting of astrocyte elevated gene-1 (AEG-1), enhancer of zeste homolog 2 (EZH2) and GAPDH, rabbit EZH2 antibody (#5246) was purchased from Cell Signaling Technology, Inc., and rabbit AEG-1 antibody (abs124058) and mouse GAPDH antibody (ab127428) were obtained from Absin Bioscience, Inc. (Shanghai, China). Western blotting was performed as previously described (19 (link)).

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Li X., Zhao X., Yang B., Li Y., Liu T., Pang L., Fan Z., Ma W., Liu Z, & Li Z. (2018). Long non-coding RNA HOXD-AS1 promotes tumor progression and predicts poor prognosis in colorectal cancer. International Journal of Oncology, 53(1), 21-32.

Publication 2018

AEG-1

Antibody Astrocyte Enhancer of zeste homolog 2 Gapdh Gene Mouse Rabbit

Corresponding Organization :

Other organizations : Second Affiliated Hospital of Xi'an Jiaotong University

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Variable analysis

independent variables

Rabbit EZH2 antibody (#5246) from Cell Signaling Technology, Inc.
Rabbit AEG-1 antibody (abs124058) from Absin Bioscience, Inc. (Shanghai, China)
Mouse GAPDH antibody (ab127428) from Absin Bioscience, Inc. (Shanghai, China)

dependent variables

Expression levels of astrocyte elevated gene-1 (AEG-1), enhancer of zeste homolog 2 (EZH2) and GAPDH

control variables

Western blotting protocol as previously described (19 (link))

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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