Apoptotic Signaling Pathway Analysis

Western Blot analysis was performed as described before^{35 (link)}. In brief, cells were homogenized in RIPA lysis buffer supplemented with protease inhibitor cocktail and phosphatase inhibitor cocktail (Sigma, St. Louis, MO). Protein lysates were resolved on SDS-PAGE gels before being transferred to the PVDF membrane. Anti-FADD, anti-Caspase-8, anti-RIP, and anti-RIP3 antibodies were purchased from Santa Cruz (sc-271748, sc-56070, sc-133102, and sc-374639 respectively). Mouse monoclonal anti-Actin antibody was used as a loading control. The densities of bands were quantitated using ImageJ software.

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Carnino J.M., Lee H., He X., Groot M, & Jin Y. (2020). Extracellular vesicle-cargo miR-185-5p reflects type II alveolar cell death after oxidative stress. Cell Death Discovery, 6, 82.

Publication 2020

protease inhibitor cocktail phosphatase inhibitor cocktail anti-Caspase-8 sc-271748 sc-56070 sc-133102 sc-374639

Actin Anti antibodies Anti antibody Buffer Caspase 8 Cells Fadd Gels Membrane Mouse Phosphatase Protease inhibitor Protein Pvdf Rip3 Ripa Sds page Western blot analysis

Corresponding Organization :

Other organizations : Boston University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Densities of FADD, Caspase-8, RIP, and RIP3 protein bands

control variables

Actin protein as a loading control

controls

Positive control: None mentioned
Negative control: None mentioned

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