Lung Tissue Analysis for Apoptosis and NLRP3

Lung tissue was cut to 4 microns after fixation and embedding. During the experiment, try to ensure that the slices are not contaminated, and at the same time, ensure the storage time of the samples, so that they can be processed in time and follow-up experiments can be carried out in time. According to a previous study [26 (link)], the sections were dewaxed and stained with hematoxylin and eosin to observe lung pathological changes. The sections were stained according to the experimental steps indicated by TUNEL kit (Thermo Fisher Scientific, UA) to observe the death of apoptotic cells. In addition, the expression of NLRP3 in Sample tissues was assessed by staining for NLRP3 using immunohistochemical methods.