FRET Peptide Substrates for ADAM Proteases

The FRET (Fluorescence-Resonance Energy Transfer) peptide substrates PEPDAB005, PEPDAB008, PEPDAB010, PEPDAB011, PEPDAB013, PEPDAB014 and PEPDAB022, which are based on the cleavage sites in specific proteins known to be processed by different ADAM family members 45 (link), 46 (link), were obtained from BioZyme Inc (Apex, NC). Recombinant ADAM10 and ADAM17 were purchased from R&D Systems (Minneapolis, MN). EDTA-free non-MP protease inhibitor cocktails, tablets and Halt solution, were obtained from Roche Applied Science (Indianapolis, IN) and ThermoFisher Scientific (Pittsburgh, PA), respectively. Trypsin and enzyme-free cell-dissociation solution were obtained from GIBCO-Life Technologies (Grand Island, NY).

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Yoneyama T., Gorry M., Sobo-Vujanovic A., Lin Y., Vujanovic L., Gaither-Davis A., Moss M.L., Miller M.A., Griffith L.G., Lauffenburger D.A., Stabile L.P., Herman J, & Vujanovic N.L. (2018). ADAM10 Sheddase Activity is a Potential Lung-Cancer Biomarker. Journal of Cancer, 9(14), 2559-2570.

Publication 2018

Recombinant ADAM10 ADAM17 Trypsin enzyme-free cell-dissociation solution

Adam17 Cell Cleavage Edta Enzyme Family members Fluorescence resonance energy transfer Peptide Protease inhibitor Proteins Trypsin

Corresponding Organization : UPMC Hillman Cancer Center

Other organizations : VA Pittsburgh Healthcare System, BioZyme (United States), Massachusetts Institute of Technology, Center for Systems Biology, Harvard University, Massachusetts General Hospital

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Variable analysis

independent variables

FRET (Fluorescence-Resonance Energy Transfer) peptide substrates PEPDAB005, PEPDAB008, PEPDAB010, PEPDAB011, PEPDAB013, PEPDAB014 and PEPDAB022

dependent variables

Not explicitly mentioned

control variables

Recombinant ADAM10 and ADAM17 enzymes
EDTA-free non-MP protease inhibitor cocktails
Trypsin
Enzyme-free cell-dissociation solution

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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