The pellet was resuspended in 100 μL of 6 % Chelex®100 (Bio-Rad Laboratories, Inc., USA). The suspension was incubated for 15 min at 56 °C and 1400 rpm, and subsequently heated at 99 °C for 8 min again at 1400 rpm. Both incubation steps were performed in a thermomixer. Finally, the samples were centrifuged at 16000×g for 2 min to precipitate food leftovers, cellular debris, and the resin.
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