A tissue microarray containing a total of 77 prostate specimens was used (Supplementary Table 2 ). The tissue microarray was obtained from Provitro (Berlin, Germany; Cat #401 2209, Lot #146.1 P020212, 26–46). Detailed information on all cancer specimens can be found at http://www.provitro.com/fileadmin/provitro-data/TMA/4012209.pdf . Immunostaining was evaluated using a previously described scoring method (Kojima et al, 2012 (link)). Tissue microarray was immunostained with an Ultra-Vision Detection System (Thermo Scientific, Fremont, CA, USA) following the manufacturer's protocol. Primary rabbit polyclonal antibodies against WWP1 (1:200, ab43791; Abcam) were used for immunochemistry. The slides were treated with biotinylated goat antibodies (Histofine SAB-PO kit; Nichirei, Tokyo, Japan).
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Prostate Tissue Microarray Immunostaining
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Other organizations : Chiba University, Teikyo University Chiba Medical Center, Kagoshima University
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- Tissue microarray containing a total of 77 prostate specimens
- Immunostaining score of WWP1 expression
- Tissue microarray obtained from Provitro (Berlin, Germany; Cat #401 2209, Lot #146.1 P020212, 26–46)
- Immunostaining evaluated using a previously described scoring method (Kojima et al, 2012)
- Tissue microarray immunostained with an Ultra-Vision Detection System (Thermo Scientific, Fremont, CA, USA) following the manufacturer's protocol
- Primary rabbit polyclonal antibodies against WWP1 (1:200, ab43791; Abcam) used for immunochemistry
- Slides treated with biotinylated goat antibodies (Histofine SAB-PO kit; Nichirei, Tokyo, Japan)
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