Baby hamster kidney (BHK-21) cells (ATCC CCL-10) were maintained in Eagle's minimum essential medium (EMEM) (Wako Pure Chemical Industries, Ltd., Osaka, Japan) containing 5% fetal bovine serum (FBS) (Equitech-Bio, Kerrville, TX, USA) and 1% antibiotic/antimycotic (Nacalai Tesque, Kyoto, Japan). The cells were maintained at 37°C under 5% CO2 until use.
The LGTV TP21 used in this study was amplified in BHK cells, and the virus stock titer was determined via focus forming assay as previously described (Talactac et al., 2016 (link)). The virus stock was aliquoted and stored at −80°C until use.
The LGTV TP21 used in this study was amplified in BHK cells, and the virus stock titer was determined via focus forming assay as previously described (Talactac et al., 2016 (link)). The virus stock was aliquoted and stored at −80°C until use.
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