We selected four study subjects enrolled in the Northwestern University Clinical Research Site for the MWCCS. The four men were well-suppressed patients who had received antiretroviral drugs for at least 5 years. Subject A received stavudine, tenofovir, lamivudine, and nevirapine; subject B received efavirenz, lopinavir, and tenofovir; subject C received ritonavir, amprenavir, and lamivudine/zidovudine; and subject D received indinavir and lamivudine at the sampling visits. We obtained PBMCs from cryostorage more than 5 years after starting antiretroviral therapy and undetectable plasma HIV-1 (<50 copies/ml). Laboratory procedures for clinical sample management are described elsewhere (82 (link)). The Institutional Review Board of Northwestern University approved the study (STU00022906-CR0008) with most recent approval date of 16 May 2022. All participants provided written informed consent.