Thyroid Molecular Signaling Pathway

ZY-444 was a gift from Professor Yihua Chen’s group at East China Normal University, Shanghai, China [17 (link)]. SCH772984 (ERK1/2 inhibitor, S7101) was purchased from Selleck, Shanghai, China. All drugs were dissolved in dimethyl sulfoxide solution.
The antibodies used in this study were: anti-PC (Santa Cruz Biotechnology, USA, sc-271493), anti-TSHR (Beyotime, AF1186), anti-NIS (Invitrogen, MA5-12308), anti-TPO (Abcam, UK, ab133322), anti-TG (Santa Cruz Biotechnology, sc-365997), anti-phospho-ERK1/2 (Cell Signaling Technology, USA, 4370), anti-ERK1/2 (Cell Signaling Technology,4695), anti-phospho-MEK1/2 (Cell Signaling Technology, 9154), anti-MEK1/2 (Cell Signaling Technology, 4694), anti-GAPDH (Beyotime, AF1186), and anti-α-tubulin (Beyotime, AF5012).