After anesthesia of the animal using a combination of ketamine and xylazine, the surgical site on the animal’s chest was disinfected with 70% alcohol. After keeping the animal fixed on the operating desk, using an otoscope number 3 and a green angiocatheter, the animal was intubated and connected to a ventilator (inter med Bear) (inhaul to exhalation ratio of 1 to 2 and 80–90 breaths per minute with a volume of 8 ml). In the space between the third and fourth ribs, the chest was cut to a length of 10 mm. With this incision, the LAD vessel was identified as a bright red pulsating spike that flows in the middle of the heart wall from under the left atrium to the apex of the heart. The LAD vessel was closed with the help of 0.6 mm polypropylene suture 1–2 mm below the level of the tip of the left atrium and was completely closed by tying two knots at this point. Left ventricular anterior wall infarction was confirmed by sudden myocardial coloration (discoloration). An increase in ST was also observed after ligation. Then, the chest, muscle layers, and skin were sewn in three layers using 0.5 proline suture and the animal's skin was sutured with 0.3 proline suture. When the rats regained consciousness, they were removed from the ventilator. After 48 h, the rats were anesthetized again and with echo vivid7 probe 10 s (MHz), an echo was performed to determine MI. In addition, cefazolin and tramadol as antibiotics and analgesics were injected twice a day, 1 day before surgery and 3 days after surgery. Rats in the MI group underwent all surgeries without occlusion of the left coronary artery. Also, Ct group rats did not receive any intervention and were kept only in the laboratory.
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