A selection of
sublethal end points related to, e.g., neurological impacts, lipid
metabolism, and oxidative responses of shrimp were addressed in the
study. Validated protocols were used to analyze the following parameters:
acetylcholinesterase activity (AChE) in gills and muscle tissues to
assess neurotoxicity; AcylCoA (acyl coenzyme A) oxidase activity (ACOX),
involved in different aspects of lipid homeostasis in the digestive
gland; antioxidant response and oxidative damage in digestive gland
by total oxyradical scavenging capacity (TOSC assay toward peroxyl
and hydroxyl radicals); and lipid peroxidation (malondialdehyde levels).
The parameters described above were analyzed in tissues at the end
of exposure (day 4) and at the end of the recovery period (day 14).
Analytical methods are described in theSI .
sublethal end points related to, e.g., neurological impacts, lipid
metabolism, and oxidative responses of shrimp were addressed in the
study. Validated protocols were used to analyze the following parameters:
acetylcholinesterase activity (AChE) in gills and muscle tissues to
assess neurotoxicity; AcylCoA (acyl coenzyme A) oxidase activity (ACOX),
involved in different aspects of lipid homeostasis in the digestive
gland; antioxidant response and oxidative damage in digestive gland
by total oxyradical scavenging capacity (TOSC assay toward peroxyl
and hydroxyl radicals); and lipid peroxidation (malondialdehyde levels).
The parameters described above were analyzed in tissues at the end
of exposure (day 4) and at the end of the recovery period (day 14).
Analytical methods are described in the
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