Transcriptional Response to Oxidative Stress

When the bacteria were cultured in TSB medium to OD₆₀₀ 1.0, they were treated in PBS containing 0.4 mM H₂O₂ for 10 min and collected by centrifugation. Total RNA was extracted using RNAprep Pure Cell/Bacteria Kit (Tiangen Biotech, Beijing, China). Then, cDNA was synthesized with 1 μg RNA and PrimeScript™ IV 1st strand cDNA Synthesis Mix (Takara, Dalian, China). For the qRT-PCR, cDNA was mixed with specific primers (Table 2) and chamQ universal SYBR quantitative RT-PCR master mix (Vazyme, Nanjing, China). 16S rRNA gene was used as internal controls.

Free full text: Click here

Fu T., Fan Z., Li Y., Li Z., Du B., Liu S., Cui X., Zhang R., Zhao H., Feng Y., Xue G., Cui J., Yan C., Gan L., Feng J., Xu Z., Yu Z., Tian Z., Ding Z., Chen J., Chen Y, & Yuan J. (2023). ArcR contributes to tolerance to fluoroquinolone antibiotics by regulating katA in Staphylococcus aureus. Frontiers in Microbiology, 14, 1106340.

Publication 2023

RNAprep Pure Cell/Bacteria Kit PrimeScript™ IV 1st strand cDNA Synthesis Mix

16s rrna Bacteria Cdna Cell Centrifugation Cultured medium Gene H2o2 Primers Rrna gene Rt pcr Synthesis

Corresponding Organization : Capital Institute of Pediatrics

Other organizations : University of Science and Technology of China

Top 5 similar protocols

Variable analysis

independent variables

Treating bacteria with 0.4 mM H2O2 for 10 min

dependent variables

Total RNA extracted
CDNA synthesized from 1 μg RNA
Gene expression quantified by qRT-PCR

control variables

Culturing bacteria in TSB medium to OD600 1.0
Using 16S rRNA gene as internal controls for qRT-PCR

positive controls

None specified

negative controls

None specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!