Profiling Cell Growth and Proliferation

The absorbance value of BC cells at the wavelength of 450 nm was recorded using CCK-8 at the indicated time points to plot the growth curves. In terms of the colony formation assay, BC cells (1000 cells/well) were seeded into 6-well plates, which were then routinely cultured for two weeks before their fixed staining. Cells were stained using EdU reagent and 4',6-diamidino-2-phenylindole (DAPI) to capture the cell proliferation signals under a fluorescence microscope (Leica, Wetzlar, Germany).

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Wang B., Chen H., Deng Y., Chen H., Xing L., Guo Y., Wang M, & Chen J. (2023). CircDNAJC11 interacts with TAF15 to promote breast cancer progression via enhancing MAPK6 expression and activating the MAPK signaling pathway. Journal of Translational Medicine, 21, 186.

Publication 2023

fluorescence microscope

Assay Cck 8 Cell proliferation Cells Fluorescence microscope

Corresponding Organization :

Other organizations : Chongqing Medical University, First Affiliated Hospital of Chongqing Medical University

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Variable analysis

independent variables

Time points indicated for absorbance measurements using CCK-8 assay
Cell seeding density (1000 cells/well) for colony formation assay

dependent variables

Absorbance values at 450 nm wavelength (using CCK-8 assay)
Colony formation (number of colonies formed)

control variables

Cell type (BC cells)
Culture conditions (routine culturing for two weeks)
Staining method (EdU and DAPI)
Fluorescence microscope (Leica, Wetzlar, Germany)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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