Flow cytometry was performed as previously described (10 (link)). All of the conjugated antibodies were purchased from eBioscience and detailed information is given in Supplementary Table S2. Fc Blocking Ab (catalog # 14–9161–73), anti-mouse CD16/CD32 (catalog # 14–0161–82), fixation/permeabilization concentrate (catalog # 00–5123–56), fixation/permeabilization diluent (catalog # 00–5223–56), and permeabilization buffer (catalog # 00–8333–56) were also purchased from eBioscience. Cytometry data were acquired on a BD LSRFortessa 6-Laser flow cytometer and analyzed on FlowJo 6 software. This work was supported by the Northwestern University – Flow Cytometry Core Facility supported by NCI CA060553.
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Flow Cytometry Analysis Protocol
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Corresponding Organization : Neurological Surgery
Other organizations : Loyola University Chicago, Loyola University Medical Center, University of Manchester, University of Tübingen, UPMC Hillman Cancer Center, University of Pittsburgh
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Variable analysis
independent variables
- Not explicitly mentioned
- Cytometry data acquired on a BD LSRFortessa 6-Laser flow cytometer
- Analyzed on FlowJo 6 software
- Flow cytometry was performed as previously described (10 (link))
- All of the conjugated antibodies were purchased from eBioscience and detailed information is given in Supplementary Table S2
- Fc Blocking Ab (catalog # 14–9161–73), anti-mouse CD16/CD32 (catalog # 14–0161–82), fixation/permeabilization concentrate (catalog # 00–5123–56), fixation/permeabilization diluent (catalog # 00–5223–56), and permeabilization buffer (catalog # 00–8333–56) were also purchased from eBioscience
- Not explicitly mentioned
- Not explicitly mentioned
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