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Generation and Characterization of FcγRIIA Mutants

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Okubo K., Brenner M.D., Cullere X., Saggu G., Patchen M.L., Bose N., Mihori S., Yuan Z., Lowell C.A., Zhu C, & Mayadas T.N. (2021). Inhibitory affinity modulation of FcγRIIA ligand binding by glycosphingolipids by inside-out signaling. Cell reports, 35(7), 109142.

Publication 2021

HEK293T cells Lipofectamine BD FACS Aria TRCN0000230901 SHC001 SHC002

Aria Cell lines Cells Cytoplasmic Dimethylformamide Fcγriia Hl60 cells Human Itam Jurkat cells L glutamine Lipofectamine Mutations Penicillin Plasmids Populations Puromycin Shrna Site directed mutagenesis Streptomycin Tail

Corresponding Organization : Harvard University

Other organizations : Georgia Institute of Technology, Biota Pharmaceuticals (United States), University of California, San Francisco

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