Double-stranded cDNA of eight human tissues (brain, heart, kidney, testis, liver, spleen, lung, and skeletal muscle) were generated with the Marathon cDNA amplification kit (Clontech). The cDNA concentration was normalized by quantitative PCR against AGPAT1 and EEF1A1 genes. The PCRs were performed in 386-well plates in a total volume of 12.5 μLl. One microliter of normalized cDNA was mixed with JumpStart REDTaq ReadyMix (Sigma) and primers (4 μM) with a Freedom evo robot (TECAN). The 10 first cycles of amplification were performed with a touchdown annealing temperature decreasing 1°C per cycle from 65°C to 55°C; annealing temperature of the next 30 cycles was carried out at 55°C. For each tissue, 2 μL of each RT-PCR reaction were pooled together and purified with the QIAquick PCR purification Kit (Qiagen) according to the manufacturer's recommendations. This purified DNA was directly used to generate a sequencing library with the “Genomic DNA sample prep kit” (Illumina) according to the manufacturer's recommendations with the exclusion of the fragmentation step. This library was subsequently sequenced on an Illumina Genome Analyzer 2 platform.