Immunoblotting Analysis of Key Signaling Proteins

This has been performed as previously described [34 (link)]. Antibodies directed against alpha smooth muscle actin (α-SMA), transforming growth factor beta 1 (TGF-β1, 2AR2), Stromal-derived factor-1 (SDF-1), AUF1 (ab50692) and IL-6 were purchased from Abcam (Cambridge, MA); Lin28B (D4H1), NF-kB (p50), NF-kB (p65) (D14E12), AKT (C73H10), p-AKT (Thr308), STAT3 and pSTAT3-Tyr705 (D3A7) from Cell Signaling (Danvers, MA); p16^INK4a from BD Biosciences (San Jose, CA); p21 (F-5), PTEN (A2B1) and Glyceraldehydes-3-phosphate dehydrogenase (GAPDH, FL-335) were purchased from Santa Cruz (Santa Cruz, CA).

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Hendrayani S.F., Al-Harbi B., Al-Ansari M.M., Silva G, & Aboussekhra A. (2016). The inflammatory/cancer-related IL-6/STAT3/NF-κB positive feedback loop includes AUF1 and maintains the active state of breast myofibroblasts. Oncotarget, 7(27), 41974-41985.

Publication 2016

IL-6 pSTAT3-Tyr705 (D3A7) p16INK4a PTEN (A2B1)

Alpha actin Antibodies Dehydrogenase Factor 1 Gapdh Nf kb P16ink4a Phosphate Pten Smooth muscle Stat3 Tgf β1 Transforming growth factor beta 1

Corresponding Organization : King Faisal Specialist Hospital & Research Centre

Other organizations : King Saud University, Instituto de Biologia Experimental e Tecnológica

Top 5 similar protocols

Variable analysis

independent variables

Antibodies directed against alpha smooth muscle actin (α-SMA)
Antibodies directed against transforming growth factor beta 1 (TGF-β1, 2AR2)
Antibodies directed against Stromal-derived factor-1 (SDF-1)
Antibodies directed against AUF1 (ab50692)
Antibodies directed against IL-6
Lin28B (D4H1)
NF-kB (p50)
NF-kB (p65) (D14E12)
AKT (C73H10)
P-AKT (Thr308)
STAT3
PSTAT3-Tyr705 (D3A7)
P16INK4a
P21 (F-5)
PTEN (A2B1)

dependent variables

Not explicitly mentioned

control variables

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH, FL-335)

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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