The studied dataset comprised 68 mature cells taken from CAUP K608, a clonal strain of Micrasterias compereana. This strain, which has been used as a holotype for the taxonomic description of the species by Neustupa et al. [46 (link)], was originally isolated in 2011 from oligotrophic peaty pools near Étang Hardy, Aquitaine, France (43°43′08.60″N, 01°22′09.42″W). It was cultivated in 250 ml Erlenmeyer flasks with approximately 125 ml of the MES-buffered DY IV liquid medium at 22 °C and illuminated at 40 μmol photons m−2 s−1 with 18 W cool fluorescent tubes (Philips TLD 18 W/33), at a light:dark (L:D) regime of 12:12 h.
The cells were photographed at 200× magnification on an Olympus BX51 light microscope with Olympus DP27 digital photographic equipment. In total, 208 structurally corresponding landmarks were depicted on the front-view images of the cells (Fig.1b , Additional file 1 ) using TpsDig software, ver. 2.15 [47 ]. To assess the measurement error, all landmarks were digitised twice. In the first digitisation, the landmarks were registered clockwise starting from the left margin of the cellular isthmus. Conversely, the second digitisation proceeded counter clockwise from the same starting point and the landmarks were relabelled to match the labels of the first digitisation.
The cells were photographed at 200× magnification on an Olympus BX51 light microscope with Olympus DP27 digital photographic equipment. In total, 208 structurally corresponding landmarks were depicted on the front-view images of the cells (Fig.
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