Dengue Infection: Mouse Model and Interventions

For infections, DENV serotype 2, strain Eden2, was used. This clinical isolate was derived from the same study (Early Dengue Infection and Outcomes Study, Eden ; Low et al., 2006 (link)) as the patient sera in Figure 6. The culture conditions we used to maintain this strain and its interactions with MCs were previously described (St John et al., 2011 (link)). Mouse infections were performed by intra-peritoneally injection of 1 × 10⁶ PFU (WT and Sash mice) or 2 × 10⁵ PFU (IFN-α,β,γ-R^−/− mice). Cromolyn (3 mg/mouse/day) and ketotifen (0.6 mg/mouse/day) (both from Sigma) were injected intra-peritoneally in PBS as vehicle either 1 hr (WT and Sash mice) or 24 hr (IFN-α,β,γ-R^−/− mice) after infection. Montelukast (brand Singulair; Merck) was administered (0.4 mg/mouse) by oral gavage 1 hr after infection by crushing tablets using a mortar and pestle and resuspending in PBS.