Wheat arabinoxylan (WAX) was of medium viscosity and supplied by Megazyme (Wicklow, Ireland). The chemical composition of this type of WAX has previously been described [17 (link)]. Beechwood xylan (BeWX) and all chemicals were purchased from Sigma-Aldrich (St Louis, MO, USA) unless otherwise specified. AaXynA was kindly provided by the laboratory of Food Chemistry (Wageningen University) as described elsewhere [8 (link)].
Luria-Bertani (LB) medium contained per L: 10 g NaCl, 10 g tryptone (Oxoid), 5 g yeast extract (Roth). LB2 contains per liter: 10 g tryptone (Oxoid), 5 g yeast extract (Roth), 10 g sodium chloride and salts mix consisting of 1 g NH4Cl; 3 g NaCl; 1.50 g Na2SO4; 0.08 g NaHCO3; 1 g KCl; 1.8 g MgCl2 × 6H2O; 0.30 g CaCl2 × 2H2O. pH was set to 6.6 at room temperature and the medium was autoclaved for 20 min at 121 °C, after which 1 mL K2HPO4 (250 g/L) was added.
For plasmid propagation and protein expression E. coli strains DH5α and BL21(DE3) were used respectively. E. coli strains were grown in LB medium at 37 °C, pH 7.0 with an agitation speed of 150 RPM.
Geobacillus thermodenitrificans T12 was isolated from compost [18 (link)]. Strain T12 was grown in LB2 medium at 65 °C, 150 RPM and pH 7.0.
Luria-Bertani (LB) medium contained per L: 10 g NaCl, 10 g tryptone (Oxoid), 5 g yeast extract (Roth). LB2 contains per liter: 10 g tryptone (Oxoid), 5 g yeast extract (Roth), 10 g sodium chloride and salts mix consisting of 1 g NH4Cl; 3 g NaCl; 1.50 g Na2SO4; 0.08 g NaHCO3; 1 g KCl; 1.8 g MgCl2 × 6H2O; 0.30 g CaCl2 × 2H2O. pH was set to 6.6 at room temperature and the medium was autoclaved for 20 min at 121 °C, after which 1 mL K2HPO4 (250 g/L) was added.
For plasmid propagation and protein expression E. coli strains DH5α and BL21(DE3) were used respectively. E. coli strains were grown in LB medium at 37 °C, pH 7.0 with an agitation speed of 150 RPM.
Geobacillus thermodenitrificans T12 was isolated from compost [18 (link)]. Strain T12 was grown in LB2 medium at 65 °C, 150 RPM and pH 7.0.
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