Enzymatic activities of the Rubisco and PEPC enzymes were measured as described previously (Cousins et al., 2007 (link); Pengelly et al., 2010 (link)) with some modifications. The activity of both enzymes was calculated by monitoring the decrease of NADH absorbance at 340 nm with a spectrophotometer (Unico UV-2802PC, USA).
Immunoblotting was performed according to Cousins et al. (2007) (link) with some modifications. After SDS–PAGE, the gels were subjected to immunoblotting using antisera including anti-Rubisco large subunit-RBCL (Agrisera, Sweden, product no. AS03 037), anti-Rubisco small subunit RBCS (Agrisera product no. AS07 259), or anti-PEPC (Agrisera product no. AS09 458) of Arabidopsis thaliana. The total soluble protein content was determined according to Bradford (1976) (link).
Immunoblotting was performed according to Cousins et al. (2007) (link) with some modifications. After SDS–PAGE, the gels were subjected to immunoblotting using antisera including anti-Rubisco large subunit-RBCL (Agrisera, Sweden, product no. AS03 037), anti-Rubisco small subunit RBCS (Agrisera product no. AS07 259), or anti-PEPC (Agrisera product no. AS09 458) of Arabidopsis thaliana. The total soluble protein content was determined according to Bradford (1976) (link).
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