Generation and Characterization of Angptl2 and Mag Knockout Mice

The Angptl2 knockout mice (Angptl2^fl/fl) with insertion of loxp in flanks of exon 2 in C57BL/6 background were generated by Nanjing Mouse Model, Lt Corporation. The male Angptl2^fl/fl; stra8-cre mice (Angptl2 is specifically deleted in early-stage spermatogonia, spermatocytes and sperm) were crossed with wild-type (WT) female mice to generate Angptl2 knockout mice (Angptl2^−/−). The genetic deletion of Angptl2 was further confirmed by PCR. The Mag knockout mice (Mag^−/−) in C57BL/6 background were purchased from Mutant Mouse Regional Resource Centers (MMRRC). Mag^−/−Angptl2^−/− double knockout mice and their littermates Mag^−/−Angptl2^+/+ mice were also bred for the related experiments. The Guideline for Animal Care at Shanghai Jiao Tong University School of Medicine approved all the animal experimental procedures.