SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) was prepared using polyacrylamide gel premix (MDBio, China), with 5% stacking gel and 15% separating gel. After electrophoresis, the gel was stained with Coomassie Brilliant Blue R250 solution or transferred to polyvinylidene fluoride membrane for Western blot detection.
Native-PAGE (native polyacrylamide gel electrophoresis) was prepared using polyacrylamide gel premix (MDBio, China), with a stacking gel of 6% and a separating gel of 8%. After electrophoresis, it was stained with Coomassie Brilliant Blue R250 solution.
In western blot detection, to detect the protein expression, the primary antibody was histidine-tag antibody (Sangon Biotech, China), secondary antibody peroxidase-conjugated goat anti-mouse IgG (Sangon Biotech, China); and to specifically detect the target phycobiliprotein, the primary antibody was phycocyanin and allophycocyanin specific antibody (Sino Biological Inc., China), and the secondary antibody was peroxidase-conjugated goat anti-rabbit IgG (Sangon Biotech, China). After hybridization, DAB solution (MDBio, China) was used for color development.
Native-PAGE (native polyacrylamide gel electrophoresis) was prepared using polyacrylamide gel premix (MDBio, China), with a stacking gel of 6% and a separating gel of 8%. After electrophoresis, it was stained with Coomassie Brilliant Blue R250 solution.
In western blot detection, to detect the protein expression, the primary antibody was histidine-tag antibody (Sangon Biotech, China), secondary antibody peroxidase-conjugated goat anti-mouse IgG (Sangon Biotech, China); and to specifically detect the target phycobiliprotein, the primary antibody was phycocyanin and allophycocyanin specific antibody (Sino Biological Inc., China), and the secondary antibody was peroxidase-conjugated goat anti-rabbit IgG (Sangon Biotech, China). After hybridization, DAB solution (MDBio, China) was used for color development.
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