SARS-CoV-2 Spike Protein Purification

Purified SARS-CoV-2 spike antigen (S1-RBD) [His-Tag (HEK293)] was purchased from the Native Antigen Company [REC31882-500].
Tris-HCl was from Invitrogen. Dithiothreitol (DTT), iodoacetamide (IAM) and urea were from Sigma-Aldrich, digestion enzymes endoproteinase Lys-C and chymotrypsin from Thermo Scientific and MS grade formic acid and acetonitrile from Biosolve.

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Frans G., Dillaerts D., Dehaemers T., Van Elslande J., De Leeuw J., Boon L., Maes W., Callewaert N., Calcoen B., Ancheva L., Cockx M., Geukens N., Arat K., Derua R., Vermeersch P., Carpentier S.C, & Bossuyt X. (2023). Complementarity determining regions in SARS-CoV-2 hybrid immunity. Frontiers in Immunology, 14, 1050037.

Publication 2023

Tris-HCl urea endoproteinase Lys-C chymotrypsin

Acetonitrile Antigen Chymotrypsin Digestion Dithiothreitol Endoproteinase lys c Enzymes Formic acid Iodoacetamide Sars cov 2 Tris Urea

Corresponding Organization :

Other organizations : KU Leuven, AZ Groeninge, Research Institute for Chromatography

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Variable analysis

independent variables

Purified SARS-CoV-2 spike antigen (S1-RBD) [His-Tag (HEK293)] purchased from the Native Antigen Company [REC31882-500]

dependent variables

Not explicitly mentioned

control variables

Tris-HCl from Invitrogen
Dithiothreitol (DTT), iodoacetamide (IAM) and urea from Sigma-Aldrich
Digestion enzymes endoproteinase Lys-C and chymotrypsin from Thermo Scientific
MS grade formic acid and acetonitrile from Biosolve

controls

No positive or negative controls explicitly mentioned

Annotations

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