As described in the previous study (15 (link)), RAW264.7 cells were treated with ox-LDL at 80 μg/mL for 48 h. Oil Red O staining was performed to observe the lipid droplets in the cells. In this study, there were three treatment groups: control group (RAW264.7 cells only), model group (RAW264.7 cells treated with ox-LDL), and GXHP group (RAW264.7 cells treated with ox-LDL and GXHP at 1.8 g/L). The cells in the three groups were collected for MC-seq and RNA-seq.
Jia Z., Mei J., Zhang Y., Wang Y., Wang H., Wang A., Xu F, & Zhou Q. (2023). Whole genome methylation combined with RNA-seq reveals the protective effects of Gualou-Xiebai herb pair in foam cells through DNA methylation mediated PI3K-AKT signaling pathway. Frontiers in Immunology, 14, 1054014.
Publication 2023
Lipid droplets Ox ldl Raw264 7 cells Rna seq
Corresponding Organization : Chinese Academy of Medical Sciences & Peking Union Medical College
Other organizations :
Beijing University of Chinese Medicine
Treatment group: control group (RAW264.7 cells only), model group (RAW264.7 cells treated with ox-LDL), and GXHP group (RAW264.7 cells treated with ox-LDL and GXHP at 1.8 g/L)
dependent variables
Lipid droplets in the cells (observed via Oil Red O staining)
Transcriptome (measured via MC-seq and RNA-seq)
control variables
Cell line: RAW264.7 cells
Concentration of ox-LDL: 80 μg/mL
Duration of treatment: 48 h
positive controls
Not explicitly mentioned
negative controls
Control group (RAW264.7 cells only)
Annotations
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