Inducing DNA Damage with Ciprofloxacin

Induction of DNA-damage using ciprofloxacin was adapted from Peña et al.^{16 (link)}. Overnight cultures were subcultured in LB for 3 h at 37 °C and grown until exponential phase. The cultures were diluted to OD₆₀₀ 0.5 and 32 µg/ml ciprofloxacin (Sigma-Aldrich) was added to the cultures if not otherwise stated. Cultures were incubated for two hours at 37 °C and harvested by centrifuging appropriate cell numbers for the desired downstream analyses.

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Eggers O., Renschler F.A., Michalek L.A., Wackler N., Walter E., Smollich F., Klein K., Sonnabend M.S., Egle V., Angelov A., Engesser C., Borisova M., Mayer C., Schütz M, & Bohn E. (2023). YgfB increases β-lactam resistance in Pseudomonas aeruginosa by counteracting AlpA-mediated ampDh3 expression. Communications Biology, 6, 254.

Publication 2023

ciprofloxacin

Ciprofloxacin Dna damage

Corresponding Organization :

Other organizations : Institute of Medical Microbiology and Hygiene, University of Tübingen, German Center for Infection Research

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Variable analysis

independent variables

Concentration of ciprofloxacin (32 µg/ml)

dependent variables

Cell number (for downstream analyses)

control variables

Overnight culture subculturing in LB for 3 h at 37 °C
Growth until exponential phase
Dilution to OD600 0.5
Incubation time (2 hours at 37 °C)

controls

Not explicitly mentioned
Not explicitly mentioned

Annotations

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