Phylogenetic Analysis of Vasa Protein

Phylogenetic analysis and amino acid alignment analysis were performed using MEGA 7 [64 (link)]. For phylogenetic analysis, evolutionary history was inferred using the neighbor-joining method [65 (link)]. The percentages of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) were determined as previously described [66 (link)]. The evolutionary distances were computed using the Poisson correction method and are in units of the number of amino acid substitutions per site. Protein sequence of Vasa were downloaded from National Center for Biotechnology Information. The accession numbers are as follows: Plutella xylostella, XP_037961717.1; D. rerio, CAA72735.1; M. musculus, EDL18409.1; Tribolium castaneum, NP_001034520.2; B. mori, NP_001037347.1; Manduca sexta, NP_001037347.1; X. laevis, NP_001081728.1; C. elegans, NP_491113.1; D. melanogaster, NP_723899.1; Nasonia vitripennis, XP_001603956.3; Amyelois transitella, XP_013187571.1; Helicoverpa armigera, XP_021190483.1; Aedes aegypti, XP_021700879.1; Bicyclus anynana, XP_023939227.1; and Danaus plexippus, XP_032519693.1.

Free full text: Click here

Yang X., Chen D., Zheng S., Yi M., Wang S., Liu Y., Jing L., Liu Z., Yang D., Liu Y., Tang L., Walters J.R, & Huang Y. (2023). The Prmt5-Vasa module is essential for spermatogenesis in Bombyx mori. PLOS Genetics, 19(1), e1010600.

Publication 2023

Aedes Amino acid Amino acid substitutions C elegans D melanogaster D rerio Evolutionary Manduca sexta Musculus Protein sequence Replicate Trees Tribolium X laevis

Corresponding Organization : University of Kansas

Top 5 similar protocols

Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control, None specified
Negative control, None specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!