To recode UGA with Sec, the anticodon of allo-tRNAUTu1D in the pSecUAG plasmid (Mukai et al., 2018 (link); Chung et al., 2021 (link)) was changed to UCA. This was done using primer pair PM21/22 on the pSecUAG plasmid to generate pSecUGA.
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