Isolation of Rat Liver Mitochondria

Rat liver mitochondria were isolated according to [9 (link)] in a buffer containing 250 mM sucrose, 3 mM Tris-HCl (pH 7.3), and 0.5 mM EGTA; subsequent mitochondrial sediment was washed out twice by resuspension-centrifugation in a medium containing 250 mM sucrose and 3 mM Tris-HCl (pH 7.3) and finally suspended in 1 mL of the last buffer. According to Bradford, the mitochondrial protein content assayed was within 50–60 mg/mL.

Free full text: Click here

Korotkov S.M, & Novozhilov A.V. (2023). A Comparative Study on the Effects of the Lysine Reagent Pyridoxal 5-Phosphate and Some Thiol Reagents in Opening the Tl+-Induced Mitochondrial Permeability Transition Pore. International Journal of Molecular Sciences, 24(3), 2460.

Publication 2023

Buffer Centrifugation Egta Liver mitochondria Mitochondrial Mitochondrial protein Sucrose Tris

Corresponding Organization : Institute of Evolutionary Physiology and Biochemistry

Top 5 similar protocols

Variable analysis

independent variables

Isolation of rat liver mitochondria

dependent variables

Mitochondrial protein content

control variables

Buffer composition (250 mM sucrose, 3 mM Tris-HCl (pH 7.3), 0.5 mM EGTA)
Washing and resuspension of mitochondrial sediment in 250 mM sucrose and 3 mM Tris-HCl (pH 7.3)

controls

No explicit positive or negative controls mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!