The aligned data matrix used in the phylogenetic analyses includes a total of 180 accessions, of which 172 are ingroup corresponding to 22 (of 24) genera of the subtribe Cenchrinae [see Supporting Information Table S1]. The chloroplast DNA (cpDNA) ndhF matrix previously published [29 (link)], excluding the outgroup, was completed with 63 new sequences. Of these, we have sequenced 20 new accessions corresponding to species of the subgenera Paurochaetium and Reverchoniae of Setaria (Table S1, indicated with *) including those without a defined placement in [29 (link)] (Table S1, indicated with **) plus a second accession of Alexfloydia repens and Panicum antidotale. Eight species belonging to six closely related genera were selected as outgroup, based on [1 (link),29 (link)]: Aakia (Paspaleae, Paspalinae), Eriochloa Kunth, Moorochloa Veldkamp, Rupichloa Salariato & Morrone, Urochloa P. Beauv. (Paniceae, Melinidinae) and Panicum (Paniceae, Panicinae). Information about vouchers and accession numbers of the new sequences obtained for this study and those available in GenBank are given in Table S1.
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