Protocol detail

Histological Analysis of Glandular Tissues

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Glandular tissues were fixed in 10% buffered formalin at room temperature for 24 hours. Then glandular tissues were embedded in paraffin and serially sectioned into 4–5 μm thick paraffin sections. The sections were stained with hematoxylin-eosin (Bio Optica, Italy) using standard protocols. Sections were observed under the light microscope (Olympus BX46, Japan). The focus score in the gland was determined by the number of lymphocytic foci [21 (link)].

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Genç D., Bulut O., Günaydin B., Göksu M., Düzgün M., Dere Y., Sezgin S., Aladağ A, & Bülbül A. (2022). Dental follicle mesenchymal stem cells ameliorated glandular dysfunction in Sjögren’s syndrome murine model. PLOS ONE, 17(5), e0266137.

Publication 2022

hematoxylin-eosin

Embedded paraffin Eosin Formalin Lymphocytic number Microscope light Paraffin Tissues

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Focus score in the gland determined by the number of lymphocytic foci

control variables

Glandular tissues were fixed in 10% buffered formalin at room temperature for 24 hours
Glandular tissues were embedded in paraffin and serially sectioned into 4–5 μm thick paraffin sections
The sections were stained with hematoxylin-eosin (Bio Optica, Italy) using standard protocols

controls

No positive or negative controls were explicitly mentioned

Annotations

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