F12 and F12 (Coon’s modification) medium, latrunculin B, cycloheximide (Cat. No. C7698-1G) was from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS) and newborn calf serum were from Gemini Bio-Products (Woodland, CA, USA). Anti-α-tubulin mouse monoclonal antibodies (DM1A, Cat. No. T9026, lot no. 052M4837), anti-FLAG mouse monoclonal antibodies, were from Sigma-Aldrich. Rabbit polyclonal antibodies against full length human MAL2 (Cat. No. ab75347), and VASP (Cat. No. ab47461) were from AbCam (San Francisco, CA, USA). Rabbit polyclonal antibodies against Tks5 (Fish (M-300)) were from Santa Cruz (Dallas, TX, USA) (Cat. sc-30122). Rabbit polyclonal antibodies against Mena (Cat. No. NBP1-87914) were from NOVUS (Littleton, CO, USA). Rabbit polyclonal antibodies against rat MAL2 were generated and affinity purified as described previously [4 (link)]. Monoclonal antibodies against rat zonula occludens-1 (ZO-1) were generously provided by Dr. Ann Hubbard (Johns Hopkins University School of Medicine, Baltimore, MD, USA) and are now part of our lab stocks. Alexa488 and -568-conjugated secondary antibodies were purchased from Invitrogen Life Technologies (Carlsbad, CA, USA) and HRP-conjugated secondary antibodies were from Sigma-Aldrich.
Free full text: Click here