Two hiPSC lines (U2F and ACS-1011) used in this study were derived from healthy individuals. The U2F hiPSCs was obtained from the Cellapy Technology (Beijing, China), and the ACS-1011 was obtained from the American type culture collection (ATCC). Pluripotency and karyotype of U2F hiPSCs were confirmed by immunofluorescence and karyotype analysis as shown in our previous studies46 (link),47 (link). The hiPSCs were cultured in Matrigel (Corning, 354277)-coated plate and supplemented with the mTeSR Plus medium (STEMCELL Technologies, 05825) and 1% penicillin/streptomycin (Gibco, 10378016).
As described in our previous study46 (link), hNPCs were induced from U2F hiPSCs using the STEMdiff™ Neural Induction Medium (STEMCELL Technologies, 05835). The hNPCs were cultured in Matrigel-coated plate and maintained in the STEMdiff™ Neural Progenitor Medium (STEMCELL Technologies, 05833).
The SH-SY5Y neuroblastoma cells were cultured in high-glucose DMEM (Gibco, C11995500BT) supplemented with 10% fetal bovine serum (Gibco, A3161001C) and 1% penicillin/streptomycin.
As described in our previous study46 (link), hNPCs were induced from U2F hiPSCs using the STEMdiff™ Neural Induction Medium (STEMCELL Technologies, 05835). The hNPCs were cultured in Matrigel-coated plate and maintained in the STEMdiff™ Neural Progenitor Medium (STEMCELL Technologies, 05833).
The SH-SY5Y neuroblastoma cells were cultured in high-glucose DMEM (Gibco, C11995500BT) supplemented with 10% fetal bovine serum (Gibco, A3161001C) and 1% penicillin/streptomycin.
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