White adipose tissue was excised, fixed in formalin overnight, embedded in paraffin and sectioned. The immunofluorescence analysis of F4/80 (MCA497GA; Abd Serotec, NC, USA), lactate dehydrogenase (ab47010, Abcam)  and HIF-1α (NB100-479; Novus Biologicals, CO, USA) was conducted after deparaffinization as described previously23 (link). Sections were mounted and visualized using a Nikon Eclipse microscope.
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