Radiolabeling Procedures for PET Imaging

Unless otherwise indicated, reagents were obtained from Sigma-Aldrich and used without further purification. 8-hydroxyquinoline was obtained in ACS reagent grade, >99% purity. No-carrier-added, GMP-grade zirconium-89 (>150 MBq/nmol; BV Cyclotron, VU Amsterdam, NL) was purchased from PerkinElmer as [⁸⁹Zr]Zr-oxalate in 1 M oxalic acid. [¹¹¹In]InCl₃ in 0.1 M HCl was purchased from Curium, UK. [⁶⁸Ga] GaCl₃ was eluted with 0.1 M HCl from a TiO₂-based ⁶⁸Ge/⁶⁸Ga generator (lGG-100, Eckert & Ziegler). Copper-64 was prepared by ⁶⁴Ni(p,n)⁶⁴Cu nuclear reaction on a CTl RDS 112 11 MeV cyclotron as previously described [40 (link)]. Hydroxyethylstarch (HES200/0.5) was obtained from Carbosynth. Polysorbate 80 was obtained from Alfa Aesar. pH values were measured with a SevenCompact (Mettler-Toledo, UK) pH metre. Chemotaxis plates (polycarbonate membrane, 3.2 mm diameter, 5 μm pore size; ChemoTx® #101-5) were obtained from Neuroprobe. N- formylmethionyl-leucyl-phenylalanine (fMLP) was obtained from Santa Cruz Biotechnology. Antibodies were obtained from Miltenyi Biotec unless otherwise specified.