Dendritic Cell Microscopy Protocol

Tissue-treated 8-well chambered cover slides (Ibidi, Martinsried, Germany) were used for dendritic cells growth. The cells were cultured and fixed as previously described [19 (link)]. DAPI (4′,6-Diamidine-2′-phenylindole dihydrochloride) solution (Roche Diagnostics, Mannheim, Germany) and phalloidin Alexa Flour 488 solution (Thermo Fisher) were applied for 1 h. A Zeiss LSM510 Meta system equipped with an inverted Observer Z1 microscope and a Plan-Apochromat 63 × /1.4 oil immersion objective (Carl Zeiss MicroImaging GmbH, Göttingen, Germany) were used for confocal laser scanning microscopy.

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Ghițu A., Schwiebs A., Radeke H.H., Avram S., Zupko I., Bor A., Pavel I.Z., Dehelean C.A., Oprean C., Bojin F., Farcas C., Soica C., Duicu O, & Danciu C. (2019). A Comprehensive Assessment of Apigenin as an Antiproliferative, Proapoptotic, Antiangiogenic and Immunomodulatory Phytocompound. Nutrients, 11(4), 858.

Publication 2019

8-well chambered cover slides Observer Z1 microscope Plan-Apochromat 63 × /1.4 oil immersion objective

Cells Confocal laser scanning microscopy Dapi Dendritic cells Diagnostics Diamidine Flour Immersion Microscope Phalloidin Tissue

Corresponding Organization : Victor Babeș University of Medicine and Pharmacy Timișoara

Other organizations : Goethe University Frankfurt, University of Szeged, Spitalul Clinic Judeţean de Urgenţă "Pius Brînzeu" Timişoara

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Variable analysis

independent variables

Tissue-treated 8-well chambered cover slides (Ibidi, Martinsried, Germany)

dependent variables

Dendritic cells growth

control variables

Cells were cultured and fixed as previously described [19 (link)]
DAPI (4′,6-Diamidine-2′-phenylindole dihydrochloride) solution (Roche Diagnostics, Mannheim, Germany) and phalloidin Alexa Flour 488 solution (Thermo Fisher) were applied for 1 h
Zeiss LSM510 Meta system equipped with an inverted Observer Z1 microscope and a Plan-Apochromat 63 × /1.4 oil immersion objective (Carl Zeiss MicroImaging GmbH, Göttingen, Germany) were used for confocal laser scanning microscopy

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