Imaging and Quantification of Neuropeptide-Containing Dorsal Root Ganglion Neurons

Specimens were analyzed in a Bio-Rad Radiance Plus confocal scanning microscope (Bio-Rad, Hemel, Hempstead, UK) installed on a Nikon Eclipse E 600 fluorescence microscope (Nikon, Tokyo, Japan) and, in some experiments, an LSM 700 confocal microscope (Zeiss, Oberkochen, Germany) as in previous work [122 (link)]. The percentage of ⁺NPs in DRGs was obtained as described [115 (link)]. Four to 8 sections of each DRG from 5 animals in each group were included in the analysis. The size distribution of ⁺NPs with a visible nucleus was measured using the Nikon Eclipse E 600 fluorescence microscope with Wasabi Image Software. The ⁺NPs were divided into small, medium-sized and large according to earlier studies [53 (link)]. The relative fluorescence levels (intensity) of sst2A-like immunoreactivity (LI) in DRGs before and after nerve injury were measured as described [115 (link)] using a Sarastro 1000 confocal laser-scanning system (Molecular Dynamics, Sunnyvale, Calif., USA).

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Shi T.J., Xiang Q., Zhang M.D., Barde S., Kai-Larsen Y., Fried K., Josephson A., Glück L., Deyev S.M., Zvyagin A.V., Schulz S, & Hökfelt T. (2014). Somatostatin and its 2A receptor in dorsal root ganglia and dorsal horn of mouse and human: expression, trafficking and possible role in pain. Molecular Pain, 10, 12.

Publication 2014

LSM 700 confocal microscope Nikon Eclipse E 600 fluorescence microscope

Animals Confocal microscope Confocal scanning microscope Fluorescence Fluorescence microscope Hemel Injury Molecular dynamics Nerve Nucleus

Corresponding Organization : Harbin Institute of Technology

Other organizations : Friedrich Schiller University Jena, Jena University Hospital, Institute of Bioorganic Chemistry, Macquarie University

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Variable analysis

independent variables

Nerve injury

dependent variables

Percentage of +NPs in DRGs
Size distribution of +NPs with visible nucleus
Relative fluorescence levels (intensity) of sst2A-like immunoreactivity (LI) in DRGs

control variables

Bio-Rad Radiance Plus confocal scanning microscope
Nikon Eclipse E 600 fluorescence microscope
LSM 700 confocal microscope
Wasabi Image Software
Sarastro 1000 confocal laser-scanning system

controls

Four to 8 sections of each DRG from 5 animals in each group were included in the analysis.

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