Generating Mutant Clones in Drosophila Wing Disc

The following stocks were used: UAS-Ras^V12 (Lee et al., 1996 (link)); nub-Gal4 (Calleja et al., 1996 (link)); hid 5′F-GFP (Tanaka-Matakatsu et al., 2009 (link)); UAS-DER^DN, UAS-Diap1, UAS-puc, ap-Gal4, puc-LacZ, rpr-LacZ, and brk-LacZ (Bloomington Drosophila Stock Center); mys^RNAi, UAS-diβ (Martin-Bermudo and Brown, 1999 (link)) and hid^RNAi (Vienna Drosophila RNA-i Center). To generate mutant clones in the wing disc we used the FRT/FLP technique (Chou and Perrimon, 1992 (link)). Mutant clones were marked by the absence of GFP. The following mutant alleles and chromosomes were used: mys¹¹ [also known as mys^XG43 (Bunch et al., 1992 (link))] and e22c-Gal4 UAS-flipase (Duffy et al., 1998 (link)). The e22c-Gal4 driver is expressed in some posterior wing disc cells and was therefore used in combination with UAS- flp to generate large mys clones. Flies were raised at 25°C.