Establishing Mesothelioma Cell Lines

MeT-5A, a human immortalized mesothelial cell line, was obtained from the American Type Culture Collection (ATCC; Manassas, VA) and maintained as described [44 (link)]. Six Japanese mesothelioma cell lines, ACC-MESO-1, ACC-MESO-4, Y-MESO-8A, Y-MESO-8D, Y-MESO-9 and Y-MESO-12, were established by Yoshitaka Sekido (Aichi Cancer Center Research Institute, Nagoya, Japan) [54 (link), 55 (link)]. NCI-H290 and NCI-H2452 were kindly provided by Dr. Adi F. Gazdar (Hamon Center for Therapeutic Oncology, University of Texas Southwestern Medical Center, Dallas, TX). NCI-H2052, NCI-H2373 and MSTO-211H were purchased from ATCC. Normal human lung fibroblast (NHLF) was purchased from Lonza (Basel, Switzerland). The mesothelioma cell lines and NHLF were grown in RPMI 1640 medium, supplemented with 10% fetal bovine serum (FBS) and 1% antibiotic-antimycotic solution (Invitrogen, Carlsbad, CA). All the cell lines were cultured in a 5% CO₂ humidified atmosphere at 37 °C and used at 10-20 passages.