Continuous Epidural Delivery of XPro1595 Post-SCI

Immediately after surgery, mice were implanted with micro-osmotic pumps (Alzet model 1003D, Durect Corporation, Cupertino, CA, USA), which, for a period of three days, continuously epidurally delivered XPro1595 (2.5 ng/mL/1 μL/h, INmune Bio Inc., Monrovia, CA, USA) or saline (0.9% physiological saline/1 μL/h), as previously described [26 (link)]. The pumps were placed in such a way that the delivering end of the catheter (Micro-Renathane Tubing MRE-040, AgnTho’s AB, Lindingö, Sweden) was on top of the injured spinal cord. The catheter was sutured to the musculature, and the suture and placement of it were secured using Vetbond (3M Animal Care Products, St. Paul, MN, USA). The pump was installed in a subcutaneous pocket on the lateral back of the mouse and kept in situ throughout the entire experiment.
Mice were allowed to survive 0 h (naïve, n = 10), 1 h (n = 5/treatment group), 3 h (n = 3), 1 day (n = 15/treatment group), 3 days (n = 15/treatment group), 7 days (n = 15/treatment group), 14 days (n = 10/treatment group), 21 days (n = 13/treatment group), 24 days (n = 3/treatment group), 28 days (n = 12/treatment group), or 35-days (n = 12/treatment group) after SCI. Saline-treated mice with 3 h survival are also part of another study on inflammatory markers in SCI [6 (link)].